Found inside – Page 1215In order to prepare the inputs of the dataset, ImageJ software which is an ... Max, Perimeter, BX, BY, Angle, Circularity, Integrated Density, Skewness, ... Found inside – Page 189... bins (bin1 = pial to bin10 = ventricular) of equal size using Fiji. Integrated density was quantified per bin, and normalized for area. Found inside“The integrated density of fluorescence (IDF) was calculated employing National Institutes of Health-ImageJ software in at least four different fields per ... Found inside – Page 122Then, obtain the integrated density of signals in the image files in which background was subtracted, for each channel using ImageJ plugins including: (i) ... Found inside – Page 40The integrated density of positive staining was measured and recorded by ImageJ software on 10 slices per animal (n = 3 in each group). Found inside – Page 154Fluorescent images were taken for cell enumeration by the ImageJ (NIH). ... and their integrated density was measured by the ImageJ (NIH). Found inside – Page 443... the images and calculated with a public Java-based ImageJ program [4]. ... Integrated Density, Area, Perimeter, Minor, Circularity, Feret, MinFeret, AR, ... Found inside – Page 61The integrated density of bands were analyzed by ImageJ (D). The molecular weights of marked protein were listed followed: β-actin 45kD, OCN 50-55kD, ... Found inside – Page 37Quantification of immunofluorescence intensity was achieved with ImageJ (v1.51k). ... mean gray fluorescence value and integrated density measured. Found inside – Page 149Data were obtained from at least three independent experiments. The integrated density was measured using the ImageJ software (NIH, Bethesda, Rockville, ML, ... Found inside – Page 161The integrated density fluorescence of the nucleus was quantified using the ImageJ software and it was normalized regarding the fluorescence of the whole ... Found inside – Page 117... and through the ImageJ software by calculating the corrected total cellular fluorescence (CTCF) for each marker as integrated density–(area of selected ... Found inside – Page 2773.5.2 Measure total fluorescence intensity using ImageJ software. This is done by measuring total fluorescence intensity of the embryo (integrated density) ... Found inside – Page 235Basic image analysis includes using software, for example ImageJ, to extract integrated density information from the images.11,13 This basic approach can be ... Found inside – Page 23Absolute values of integrated pixel density (B,F) of different kinases are ... the levels of phosphorylation were quantified using ImageJ software (15) and ... Found inside – Page 39Usually, a quite low intensity of w10% laser power together with the ... Load the image sequence in ImageJ creating a stack of images using maximum or sum ... Found inside – Page 291Use ImageJ, MatLab, or other software to obtain scattering intensity tra- ... Go to Analyze, then Set Measurements, and check “Integrated Density” as the ... Found inside – Page 12176TINA software was used to quantify the intensity of the bands on the Western blots, and the ImageJ program was used to determine integrated density and maximum gray value of immunoreactions. Data analyses and statistics. Data were pro- ... Found inside – Page 243This calculation was performed using the Integrated Density function of ImageJ program (https://imagej.nih.gov/), according to Paim et al. Found inside – Page 299Images were imported into ImageJ software and converted to a 16-bit format. The parameters “area integrated intensity” and “mean gray value” were measured ... Found inside – Page 45of immunofluorescence intensity in the fluorescence images was expressed as integrated density, which was quantified using ImageJ software. The book will help readers discover the various facilities of ImageJ through a tutorial-based approach.This book is targeted at scientists, engineers, technicians, and managers, and anyone who wishes to master ImageJ for image viewing, ... Found insideThis Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. Found inside – Page 20The fluorescence emission intensity was measured in cells in at least 10 view ... using ImageJ 1.52a software by an equation: CTCF = Integrated Density ... Found inside – Page 38Integrated density of fluorescence measures the level of fluorescence intensity in the positive signal using gray pixel intensity. Found inside – Page 61In this situation , the best indication of the total amount of fluorescent probe is the integrated density or integrated intensity . This is the sum of all ... ImageJ allows rectangular , polygonal , circular , oval , or freehand ROIs . Use display zoom ( + ... Found inside – Page 226... pixel values in each image (the ImageJ software that I used to automatically do these measurements calls this 'RawIntDen' or 'Raw Integrated Density', ... Found inside – Page 9Images were processed with the software IMAGEJ 1.46r1. ... We measured area integrated density and mean gray value in every antibody-treated section. The book provides a good introduction for beginners before looking in greater detail at more advanced material in selected areas. The volume highlights the importance of technique in gathering quantitative information. Found inside – Page 64The slices were then photographed for % infarct area using ImageJ software. ... The intensity is expressed as the relative integrated density to the sham. Found insideThe text is supported by practical examples and carefully constructed chapter-ending exercises drawn from the authors' years of teaching experience, including easily adaptable Java code and completely worked out examples. Found inside – Page 181... analyzed at the same threshold intensity for all groups, was expressed as the relative integrated density of the samples relative to the control. Found inside – Page 131Therefore, each of the images were introduced into ImageJ two times; ... Instead of API, the Integrated Density ((ID), which equals fluorescent area ... Found inside – Page 190(D) Oxidation index (integrated density/area) was quantified with Oxyblot by ImageJ. The photographs represented (A) and data (B) were generated from 10 ... Found inside – Page 55These measurements were made by using ImageJ (http://imagej.nih.gov/ij/). ... groups were analyzed with ImageJ software to quantify integrated density of ... Found inside – Page 206... initially converted into a two-dimensional summation image using ImageJ, ... is then manually traced in ImageJ, and the area and integrated density in ... Found inside – Page 608... using the software package ImageJ [33]. Finally, the intensity of the image was integrated along the horizontal direction to obtain an intensity profile ... Found inside – Page 302The straight bar tool was used to plot intensity profiles of regions of interest. ... integrated density of bands after background subtraction using ImageJ. Found inside – Page 59ImageJ or Fiji can run these analyses, or the can be automated using MATLAB or LabVIEW. ... standard deviation, integrated density, skewness, and kurtosis. Found inside – Page 80... was quantified as integrated density using the ImageJ analysis software. Values were corrected by background subtraction: integrated density – (measured ... Found inside – Page 166The integrated density feature of ImageJ was used to record pixel intensities of each of these two areas . Then , the integrated density of area A minus ... Found inside – Page 73For quantification of green fluorescent protein (GFP) intensity in the facial ... Values for integrated density for each ROI were recorded and normalized to ... Found inside – Page 147... ID—the integrated density, TA—the area of the tubercle and MFB—the mean fluorescence of the background. Fluorescence intensity was also measured in ... Found inside – Page 221It can be downloaded freely using the link http://imagej.nih.gov/ij/ index.html. ... Take the average of the raw integrated density to quantify the biofilm. Found inside – Page 210Here, the whole spine is circled using the ImageJ free-hand measuring tool and then measured for area, integrated density, and mean gray value (there are ... Found inside – Page 251For this the integrated density of the Hoechst signal of single nuclei was plotted ... the area of the gap with ImageJ/FIJI software (http://fiji.sc/Fiji). Found inside – Page 143The pixel density in each spot of the array was determined by ImageJ software. ... Integrated density values were normalized to positive control spots and ... Found inside – Page 82... tools of ImageJ. Integrated density (IntDen) and area were measured. The final average fluorescent intensity per cell was calculated as IntDen/Area. Found inside – Page 133All the data were analyzed with ImageJ with integrated density. Each column in the results table calculates and displays the mean, standard deviation, ... The raw integrated density using the ImageJ ( NIH ) using ImageJ were introduced into ImageJ two ;... 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